Center for Disease Control. Overview: healthcare-associated infections. CDC. 2018. https://health.gov/hcq/prevent-hai.asp. Accessed 3 Jan 2019.
Madden GR, Weinstein RA, Sifri CD. Diagnostic stewardship for healthcare-associated infections: opportunities and challenges to safely reduce test use. J Infect Control Hosp Epidemiol. 2018;39:214–8.
Magill SS, Edwards JR, Bamberg W, Beldavs ZG, Dumyati G, et al. Multistate point-prevalence survey of health care–associated infections. N Engl J Med. 2014;370:1198–208.
Center for Disease Control. Healthcare-associated infection data 2018. CDC. 2018. https://www.cdc.gov/hai/data/index.html. Accessed 3 Aug 2019.
Umscheid CA, Mitchell MD, Doshi JA, Agarwal R, Williams K, Brennan PJ. Estimating the proportion of healthcare-associated infections that are reasonably preventable and the related mortality and cost. Infect Control Hosp Epidemiol. 2011;32:101–14. https://0-doi-org.brum.beds.ac.uk/10.1086/657912.
Kahn KL, Battles JB. Taking national action to prevent and eliminate healthcare–associated infections. Med Care. 2014. https://0-doi-org.brum.beds.ac.uk/10.1097/MLR.0000000000000083.
Sievert DM, Ricks P, Edwards JR, et al. National Healthcare Safety Network (NHSN) team and participating NHSN facilities antimicrobial-resistant pathogens associated with healthcare-associated infections: summary of data reported to the National Healthcare Safety Network at the Centers for Disease Control and Prevention 2009–2010. Infect Control Hosp Epidemiol. 2013;34:1–14.
Weiner LM, Webb AK, Limbago B, et al. Antimicrobial-resistant pathogens associated with healthcare-associated infections: summary of data reported to the National Healthcare Safety Network at the Centers for Disease Control and Prevention, 2011–2014. Infect Control Hosp Epidemiol. 2016;37(11):1288–301.
Hermandez-Jimenez E, del Rosa C, Toledano D, et al. Biofilm vs. planktonic bacteria mode of growth: which do human macrophages prefer? Biochem Biophys Res Commun. 2013;441:947–52.
Olson ME, Ceri H, Morck DW, Buret AG, Read RR. Biofilm bacteria: formation and comparative susceptibility to antibiotics. Can J Vet Res. 2002;66:86–92.
Percival S, Cutting K, Thomas J, Williams D. An introduction to the world of microbiology and biofilmology. In: Percival S, Cutting K, editors. Microbiology of wounds. Boca Raton: CRC Press; 2010.
Abdallah M, Khelissa O, Ibrahim A, Benoliel C, Heliot L, Dhulster P, Chihib NE. Impact of growth temperature and surface type on the resistance of Pseudomonas aeruginosa and Staphylococcus aureus biofilms to disinfectants. Int J Food Microbiol. 2015;214:38–47.
Macia MD, Rojo-Molinero E, Oliver A. Antimicrobial susceptibility testing in biofilm-growing bacteria. Clin Microbiol Infect. 2014;20:981–90.
Jamal M, Ahmad W, Andleeb S, Jalil F, Imran M, Nawaz MA, Hussain T, Ali M, Rafiq M, Kamil MA. Bacteria biofilms and associated infections. J Chin Med Assoc. 2017;81:7–11.
Lewoch K, Dancer SJ, Otter JA, Kerr K, et al. Beware biofilm! Dry biofilms containing bacterial pathogens on multiple healthcare surfaces; a multi-center study. J Hosp Infect. 2018;100:47–56. https://0-doi-org.brum.beds.ac.uk/10.1016/j.jhin.2018.06.028.
Gialluly DC, Morange V, Gialluly DE, Loulergue J, et al. Blood pressure cuff as a potential vector of pathogenic microorganisms: a prospective study in a teaching hospital. Infect Control Hosp Epidemiol. 2006;27:940–3.
Obasi C, Allison A, Akinpelu W, Hammons R, et al. Contamination of equipment in emergency settings: an exploratory study with a targeted automated intervention. Ann Surg Innov and Res. 2009;3:8.
Percival SL, Suleman L, Vuotto C, Donelli G. Healthcare-associated infections, medical devices and biofilms: risk, tolerance and control. J Med Microbiol. 2015;64:323–34.
Johani K, Abualsaud D, Costa DM, Hu H, et al. Characterization of microbial community composition, antimicrobial resistance and biofilm on intensive care surfaces. J Infect Pub Health. 2018;11:418–24.
Almatroudi A, Hu H, Deva A, Gosbell I, Jacombs A, et al. A new dry-surface biofilm model: an essential tool for efficacy testing of hospital surface decontamination procedures. J Microbiol Methods. 2015;117:171–6.
Hou J, Veeregowda DH, Belt-Gritter B, Busscher HJ, Mei CH. Extracellular polymeric matrix production and relaxation under fluid shear and mechanical pressure in Staphylococcus aureus biofilms. Applied and Environ Microbiol. 2018. https://0-doi-org.brum.beds.ac.uk/10.1128/AEM.01516-17.
Hu H, Johani K, Gosbell IB, Jacombs ASW, et al. Intensive care unit environmental surfaces are contaminated by multidrug-resistant bacteria in biofilms: combined results of conventional culture, pyrosequencing, scanning electron microscopy, and confocal laser microscopy. J of Hosp Infect. 2015;91:35–44. https://0-doi-org.brum.beds.ac.uk/10.1016/j.jhin.2015.05.016.
Mitchell BG, Dancer SJ, Anderson M, Dehn E. Risk of organism acquisition from prior room occupants: a systematic review and meta-analysis. J Hosp Infect. 2015;91:211–7. https://0-doi-org.brum.beds.ac.uk/10.1016/j.jhin.2015.08.005.
Chowdhury D, Tahir S, Legge S, Hu H, Prvan T, et al. Transfer of dry surface biofilm in the healthcare environment: the role of healthcare workers’ hands as vehicles. J Hosp Infect. 2018;100:85–90.
Buckinghan-Meyer K, Goeres DM, Hamilton MA. Comparative evaluation of biofilm disinfectant efficacy tests. J of Microbiol Methods. 2007;70:236–44.
Ledwoch K, Said J, Norville P, Maillard JY. Artificial dry surface biofilm models for testing the efficacy of cleaning and disinfection. Lett Appl Microbiol. 2019;68:329–36. https://0-doi-org.brum.beds.ac.uk/10.1111/lam.13143.
Ma L, Conover M, Lu H, Parsek MR, Bayles K, Wozniak DJ. Assembly and development of the Pseudomonas aeruginosa biofilm matrix; 2009. https://0-doi-org.brum.beds.ac.uk/10.1371/journal.ppat.1000354.
Moormeier DE, Bayles KW. Staphylococcus aureus biofilm: a complex developmental organism. Mol Microbiol. 2017;10:365–76.
Donskey CJ. Does improving cleaning and disinfection reduce healthcare-associated infections? American J Infect Control. 2013;41:12–9.
Almatroudi A, Gosbell IB, Hu H, Jensen O, et al. Staphylococcus aureus dry-surface biofilms are not killed by sodium hypochlorite: implications for infection control. J Hosp Infect. 2016;93:263–70. https://0-doi-org.brum.beds.ac.uk/10.1016/j.jhin.2016.03.020.
Sanchez-Vizuete P, Orgaz B, Aymerich S, et al. Pathogen protection against the action of disinfectants in multispecies biofilms. Front Microbiol. 2015. https://0-doi-org.brum.beds.ac.uk/10.3389/fmicb.2015.00705.
Stewart SP. Antimicrobial tolerance in biofilms. Microbiol Spectr. 2015. https://0-doi-org.brum.beds.ac.uk/10.1128/microbiolspec.MB-0010-2014.
Köse H, Yapar N. The comparison of various disinfectants’ efficacy on Staphylococcus aureus and Pseudomonas aeruginosa biofilm layers. Turkish J Me Sci. 2017;47:1287–94.
Otter JA, Vickery K, Walker JT, et al. Surface-attached cells, biofilms and biocide susceptibility: implications for hospital cleaning and disinfection. J Hosp Infect. 2015;89:16–27. https://0-doi-org.brum.beds.ac.uk/10.1016/j.jhin.2014.09.008.
Chen X, Stewart PS. Chlorine penetration into artificial biofilm is limited by a reaction-diffusion interaction. Environ Sci Technol. 1996;30:2078–83.
Donlan RM. Role of biofilms in antimicrobial resistance. ASAIO J. 2000;46:47–52.
Almatroudi A, Tahir S, Hu H, et al. Staphylococcus aureus dry-surface biofilms are more resistant to heat treatment than traditional hydrated biofilms. J Hosp Infect. 2018;98:161–7.
Environmental Protection Agency. EPA MLB SOP MB-19: growing a biofilm using the CDC biofilm reactor. EPA. 2017. https://www.epa.gov/sites/production/files/2017-07/documents/mb-19-may-2017.pdf. Accessed 4 Aug 2019.
ASTM International. Standard quantitative disk carrier test method for determining bactericidal, virucidal, fungicidal, mycobactericidal, and sporicidal activities of chemicals: active standard ASTM E2197. ASTM Compass. 2018. https://compass.astm.org/EDIT/html_annot.cgi?E2197+17e1 Accessed 4 Feb 2020.
Environmental Protection Agency. EPA MLB SOP MB-20: Single tube method for determining the efficacy of disinfectants against bacterial biofilm. EPA. 2017. https://www.epa.gov/sites/production/files/2017-07/documents/mb-20-may-2017.pdf. Accessed 4 Aug 2019.
Center for Disease Control and Prevention. Disinfection and Sterilization. CDC. 2019. https://www.cdc.gov/infectioncontrol/guidelines/disinfection/index.html. Accessed 4 Aug 2019.
Murtey DM, Ramasamy P. Sample preparations for scanning electron microscopy – life sciences; 2016. https://0-doi-org.brum.beds.ac.uk/10.5772/61720.
Environmental Protection Agency. Methods and guidance for testing the efficacy of antimicrobial products against biofilms on hard, non-porous surfaces. EPA. 2017. https://www.epa.gov/pesticide-analytical-methods/methods-and-guidance-testing-efficacy-antimicrobial-products-against#efficacy-data. Accessed 31 Jul 2019.
Chowdhury D, Rahman A, Hu H, Jensen SO, Deva AK, Vickery K. Effect of disinfectant formulation and organic soil on the efficacy of oxidizing disinfectants against biofilms. J Hosp Infect. 2018. https://0-doi-org.brum.beds.ac.uk/10.1016/j.jhin.2018.10.019.
Onyango LA, Alreshidi MM. Adaptive metabolism in staphylococci: survival and persistence in environmental and clinical settings. J Pathogens. 2018. https://0-doi-org.brum.beds.ac.uk/10.1155/2018/1092632.
Donlan RM. Biofilms: microbial life on surfaces. Emerg Infect Dis. 2002;8:881–90.
Meesilp N, Mesil N. Effect of microbial sanitizers for reducing biofilm formation of Staphylococcus aureus and Pseudomonas aeruginosa on stainless steel by cultivation with UHT milk. Food Sci Biotech. 2018;28:289–96.
Iibuchi R, Hara-Kudo Y, Hasegawa A, Kumagai S. Survival of Salmonella on a polypropylene surface under dry conditions in relation to biofilm-formation capability. J of Food Protec. 2010;73:1506–10.
Magrex-Debar E, Lemoine J, Gelle MP. Evaluation of biohazards in dehydrated biofilms on foodstuff packaging. Int J of Food Microbiol. 2000;55:239–43.
Marshall KC, Goodman AE. Effects of adhesion on microbial cell physiology. Colloids Surfaces B: Biointerfaces. 1994;2:1–8.
O’Connor A, McClean S. The role of universal stress proteins in bacterial infections. Curr Med Chem. 2017;24:3970–9.
Nystrom T, Olson RM, Kjellerberg S. Survival, stress resistance and alterations in protein expression in the marine Vibrio sp. strain S14 during starvation for different individual nutrients. Appl. Environ. Microbiol. 1992;58:55–65.