Table 1 Primer sequences and PCR product sizes of the florfenicol resistance genes
Primer | Sequence (5′–3′) | Amplicon size (bp) | Annealing temperature (°C) | References |
|---|---|---|---|---|
floR1-F | ATGACCACCACACGCCCCGCGTGGGC | 1198 | 58 | [7] |
floR1-R | CTTCGATCCCGCGACGTTCCTTCCGAGA | Â | Â | Â |
fexA1-F | CTCTTCTGGACAGGCTGGAA | 332 | 57 | [6] |
fexA1-R | CCAGTTCCTGCTCCAAGGTA | Â | Â | Â |
fexB1-F | ACTGGACAGGCAGGCTTAAT | 319 | 57 | [8] |
fexB1-R | CCTGCCCCAAGATACATTGC | Â | Â | Â |
cfr1-F | GGGAGGATTTAATAAATAATTTTGGAGAAACAG | 580 | 58 | [7] |
cfr1-R | CTTATATGTTCATCGAGTATATTCATTACCTCATC | Â | Â | Â |
optrA1-F | CTTATGGATGGTGTGGCAGC | 309 | 56 | [11] |
optrA1-R | CCATGTGGTTTGTCGGTTCA | Â | Â | Â |
pexA1-F | GTTGTGGTCTTTGGCCAGAG | 318 | 56 | [9] |
pexA1-R | TCCATCAAGAGGACACCACC | Â | Â | Â |