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Table 2 ESBL/pAmpC gene types and E. coli and K. pneumoniae sequence types in veterinary healthcare workers

From: ESBL/pAmpC-producing Escherichia coli and Klebsiella pneumoniae carriage among veterinary healthcare workers in the Netherlands

ESBL/pAmpC gene

Veterinary workers who work with companion animals onlya (n = 33)

Veterinary workers who work with livestock, horses or a combination of horses, livestock and/or companion animalsa (n = 14)

n (%)

ST (no. isolated)c

n (%)

ST (no. isolated)d

blaCTX-M-15

16 (48.5)

43 (1), 46 (1), 48 (1), 69 (1), 131 (4), 226 (1), 405 (1), 550 (1), 656 (1), 678 (1), 1193 (1), 1954 (1), 6438 (1), new (1)

9 (64.3)

10 (3), 95 (1), 131 (2), 394 (1), 442 (1), 1193 (1), 4684 (1)

blaCTX-M-14

6 (18.2)

38 (4), 93 (1), 744 (1)

1 (7.1)

69 (1)

blaDHA-1

4 (12.1)

10 (1), 349 (2), Kpn new (1)e

0 (0)

 

blaCTX-M-27/174b

3 (9.1)

38 (1), 131 (2)

0 (0)

 

blaCTX-M-1

2 (6.1)

69 (1), 349 (1)

0 (0)

 

blaCTX-M-55

1 (3.0)

117 (1)

1 (7.1)

744 (1)

blaCTX-M-15 and blaCMY-2

1 (3.0)

167 (1)

0 (0)

 

blaCMY-2

1 (3.0)

69 (1)

0 (0)

 

blaCTX-M-9

0 (0)

 

1 (7.1)

131 (1)

blaCTX-M-32

0 (0)

 

1 (7.1)

48 (1), 64 (1)

blaCTX-M-65

0 (0)

 

1 (7.1)

69 (1)

  1. ESBL: extended-spectrum beta-lactamase; Kpn, Klebsiella pneumoniae; pAmpC: plasmid-mediated AmpC; ST: sequence type
  2. One sequence type belongs to K. pneumoniae (ST new with pAmpC gene blaDHA-1), all other sequence types belong to E. coli
  3. aBased on frequency of contact (at least once a week)
  4. bWith the primers used no distinction could be made between blaCTX-M-27 and blaCTX-M-174
  5. cIn the isolates of one person two different ESBL-genes and STs were found (blaCTX-M-14 in ST93 and blaCTX-M-15 in ST678), and in the isolates of another person the same ESBL gene was found in two different STs (blaCTX-M-15 in ST6438 and ST1954)
  6. dIn the isolates of two persons the same ESBL gene was found in two different STs (person 1: blaCTX-M-15 in ST10 and ST4684; person 2: blaCTX-M-32 in ST48 and ST68)
  7. eAllelic profile: gapA, allele 17; infB, allele 19; mdh, allele 188; pgi, allele 20; phoE, new allele (best match with allele 545 with 1 SNP (322G → 322A)); rpoB, allele 18; tonB, allele 197. This profile is most closely related to ST2637 and ST5563