Table 2 Mutations in the quinolone resistance-determining region (QRDR) of gyrA and gyrB and fluoroquinolone MICs
Mutations in QRDR | N (%) | FQ | No. of isolates with corresponding MIC (mg/L) | ||||||
|---|---|---|---|---|---|---|---|---|---|
0.12 | 0.25 | 0.5 | 1 | 2 | 4 | 8 | |||
GyrA Asp89Asn | 2 (0.6) | MFX | 0 | 1 | 1 | 0 | 0 | 0 | 0 |
OFX | 0 | 0 | 0 | 0 | 0 | 2 | 0 | ||
LFX | 0 | 0 | 0 | 0 | 1 | 1 | 0 | ||
GyrA Ala90Val | 76 (24.4) | MFX | 18 | 3 | 16 | 23 | 8 | 8 | 0 |
OFX | 0 | 0 | 0 | 0 | 17 | 38 | 21 | ||
LFX | 0 | 0 | 0 | 0 | 56 | 20 | 0 | ||
GyrA Ser91Pro | 33 (10.6) | MFX | 3 | 9 | 10 | 4 | 7 | 0 | 0 |
OFX | 0 | 0 | 0 | 3 | 7 | 19 | 4 | ||
LFX | 0 | 0 | 0 | 4 | 25 | 4 | 0 | ||
GyrA Asp94Ala | 17 (5.5) | MFX | 0 | 0 | 10 | 4 | 3 | 0 | 0 |
OFX | 0 | 0 | 0 | 0 | 2 | 12 | 3 | ||
LFX | 0 | 0 | 0 | 0 | 15 | 2 | 0 | ||
GyrA Asp94Asn | 19 (6.1) | MFX | 0 | 0 | 4 | 4 | 7 | 4 | 0 |
OFX | 0 | 0 | 0 | 0 | 3 | 4 | 12 | ||
LFX | 0 | 0 | 0 | 0 | 4 | 15 | 0 | ||
GyrA Asp94Gly | 96 (30.9) | MFX | 0 | 0 | 10 | 14 | 44 | 28 | 0 |
OFX | 0 | 0 | 0 | 0 | 3 | 9 | 84 | ||
LFX | 0 | 0 | 0 | 0 | 11 | 85 | 0 | ||
GyrA Asp94His | 4 (1.3) | MFX | 0 | 0 | 0 | 0 | 0 | 4 | 0 |
OFX | 0 | 0 | 0 | 0 | 0 | 0 | 4 | ||
LFX | 0 | 0 | 0 | 0 | 0 | 4 | 0 | ||
GyrA Asp94Tyr | 3 (1.0) | MFX | 0 | 0 | 0 | 0 | 3 | 0 | 0 |
OFX | 0 | 0 | 0 | 0 | 0 | 0 | 3 | ||
LFX | 0 | 0 | 0 | 0 | 0 | 3 | 0 | ||
GyrA Asp94Val | 8 (2.6) | MFX | 0 | 0 | 0 | 2 | 4 | 2 | 0 |
OFX | 0 | 0 | 0 | 0 | 2 | 4 | 2 | ||
LFX | 0 | 0 | 0 | 0 | 4 | 4 | 0 | ||
GyrB Ser447Phe | 2 (0.6) | MFX | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
OFX | 0 | 0 | 0 | 0 | 0 | 2 | 0 | ||
LFX | 0 | 0 | 0 | 0 | 2 | 0 | 0 | ||
GyrB Asp461Asn | 1 (0.3) | MFX | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
OFX | 0 | 0 | 0 | 0 | 0 | 0 | 1 | ||
LFX | 0 | 0 | 0 | 0 | 0 | 1 | 0 | ||
GyrB Asp500Asn | 1 (0.3) | MFX | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
OFX | 0 | 0 | 0 | 0 | 0 | 1 | 0 | ||
LFX | 0 | 0 | 0 | 0 | 1 | 0 | 0 | ||
GyrB Ala504Thr | 3 (1.0) | MFX | 0 | 0 | 0 | 0 | 0 | 3 | 0 |
OFX | 0 | 0 | 0 | 0 | 0 | 0 | 3 | ||
LFX | 0 | 0 | 0 | 0 | 0 | 3 | 0 | ||
WT | 46 (14.7) | MFX | 0 | 7 | 15 | 12 | 8 | 4 | 0 |
OFX | 0 | 0 | 0 | 1 | 15 | 21 | 9 | ||
LFX | 0 | 0 | 1 | 3 | 24 | 16 | 2 | ||
Total | 311 (100.0) | MFX | 23 | 20 | 67 | 63 | 85 | 53 | 0 |
OFX | 0 | 0 | 0 | 4 | 49 | 112 | 146 | ||
LFX | 0 | 0 | 1 | 7 | 143 | 158 | 2 | ||