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Table 1 Primers used in this study

From: Characterisation of mobile colistin resistance genes (mcr-3 and mcr-5) in river and storm water in regions of the Western Cape of South Africa

Target gene

Primer name

DNA sequence (5′ → 3′)

Amplicon size (bp)

Reference

mcr-1

CLR5-F

5′-CGGTCAGTCCGTTTGTTC-3′

309

[1]

CLR5-R

5′-CTTGGTCGGTCTGTAGGG-3′

MCR-1YF

5′-CATATGATGATGCAGCATACTTCT GTGTGGTACCG-3′

1632

This study

MCR-1YR

5′-CTCGAGTCAGCGGATGAATGCGGT-3′

mcr-2

MCR2-IF

5′-TGTTGCTTGTGCCGATTGGA-3′

567

[2]

MCR2-IR

5′-AGATGGTATTGTTGGTTGCTG-3′

mcr-3

MCR3-F

5′-TTGGCACTGTATTTTGCATTT-3′

542

[3]

MCR3-R

5′-TTAACGAAATTGGCTGGAACA-3′

MCR-3YF

5′-ATGCCTTCCCTTATAAAAATAAA-3′

1622

This study

MCR3-YR2

5′-TCAATTATTCCGACATTGCTTA-3′

MCR-3YF2

5′-TGAAAGGGGATAAGCTGGTT-3′

Not applicable

This study

MCR-3YR3

5′-CATAATCTTGATAGTATAGTGCTG-3′

MCR-3 YF3

5′-CATATGATGCCTTCCCTTATAAAA ATAAAAATTGT-3′

1622

This study

MCR-3 YR4

5′-CTCGAGTCAATTATTCCGACATTGC-3′

mcr-4

MCR-4 FW

5′-ATTGGGATAGTCGCCTTTTT-3′

487

[36]

MCR-4 RV

5′-TTACAGCCAGAATCATTATCA-3′

mcr-5

MCR5_FW

5′-ATGCGGTTGTCTGCATTTATC-3′

1644

[5]

MCR5_REV

5′-TCATTGTGGTTGTCCTTTTCTG-3′

mcr-6

MCR-6F

5′-GTCCGGTCAATCCCTATCTGT-3′

556

[8]

MCR-6R

5′-ATCACGGGATTGACATAGCTAC-3′

mcr-7

MCR-7F

5′-TGCTCAAGCCCTTCTTTTCGT-3′

892

MCR-7R

5′-TTCATCTGCGCCACCTCGT-3′

mcr-8

MCR-8F

5′-AACCGCCAGAGCACAGAATT-3′

667

MCR-8R

5′-TTCCCCCAGCGATTCTCCAT-3′

mcr-9

MCR-9YF

5′-ATGCCTGTACTTTTCAGGGTGAAAG-3′

1619

This study

MCR-9YR

5′-TTCCGCGAATGCCGTGGCTAA-3′