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Fig. 3 | Antimicrobial Resistance & Infection Control

Fig. 3

From: Cutibacterium avidum resists surgical skin antisepsis in the groin—a potential risk factor for periprosthetic joint infection: a quality control study

Fig. 3

FISH of a C. avidum culture positive tissue (capsule tissue) biopsy fixed during surgery, shows single C. avidum cells in the tissue. The tissue section was hybridized with the panbacterial probe EUB338Cy5 (mangenta), the C. acnes/C. modestum/C. namnetense-specific probe PACFITC (green) and the C. avium/ A. thiooxidans, A. propionicum, A. jensenii, A. thoeni-specific probe PRAVCy3 (orange). Nucleic acids were stained with DAPI. a Overview of the biopsy, overlay of all fluorescence channels and background fluorescence shows the destruction of the tissue, also visible at higher magnification of the inset (b) marked in (a). c The identical microscopic field in the DAPI channel only (black and white) shows multiple DAPI-positive pleomorphic microorganisms scattered in the tissue. d Inset of (c) at higher magnification single rods are visualized in the tissue. e Some rods stained positive with PRAV in the Cy3 channel but no signal was detected with the PAC in the FITC channel (f)

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