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Table 1 Primers used in this study

From: Molecular characterization of methicillin-resistant Staphylococcus aureus clinical strains from the endotracheal tubes of patients with nosocomial pneumonia

Primer Pair

Amplified product

Sequence (5′ to 3′)

Amplicom size

Anneling temperature

References

gyrA-F

gyrA

ATG GCT GAA TTA CCT CAA TC

398 bp

55°C

13

gyrA-R

 

GTG TGA TTT TAG TCA TAC GC

   

gyrB-F

gyrB

CAGCGTTAGATGTAGCAAGC

680 bp

55°C

17

gyrB-R

 

CGATTTTGTGATATCTTGCTTTCG

   

grlA-F

grlA

CAG TCG GTG ATG TTA TTG GT

469 bp

55°C

13

grlA-R

 

CCT TGA ATA ATA CCA CCA GT

   

grlB-F

grlB

GIG AAG CIG CAC GTA A

363 bp

50°C

13

grlB-R

 

TCI GTA TCI GCA TCA GTC AT

   

ermA-F

erm(A)

TAT CTT ATC GTT GAG AAG GGA TT

138 bp

55°C

5

ermA-R

 

CTA CAC TTG GCT TAG GAT GAA A

   

ermC-F

erm(C)

CTT GTT GAT CAC GAT AAT TTC C

189 bp

55°C

5

ermCR

 

ATC TTT TAG CAA ACC CGT ATT C

   

msrA-F

msrA

TCC AAT CAT TGC ACA AAA TC

162 bp

55°C

5

msrA-R

 

AAT TCC CTC TAT TTG GTG GT

   

aac(6′)-aph(2″)F

aac(6′)-aph(2″)

TTG GGA AGA TGA AGT TTT TAG A

173 bp

55°C

5

aac(6′)-aph(2″)R

 

CCT TTA CTC CAA TAA TTT GGC T

   

tetK-F

tetK

GTA GCG ACA ATA GGT AAT AGT

360 bp

55°C

6

tetK-R

 

GTA GTG ACA ATA AAC CTC CTA

   

fusB-F

fusB

ATT CAA TCG GAA AAC TAT AAT GAT A

292 bp

60°C

21

fusB-R

 

TTA TAT ATT TCC GAT TTG ATG CAA G

   

16srRNA-F

16S rRNA

GGA GGA AGG TGG GGA TGA CG

245 bp

55°C

5

16srRNA-R

 

ATG GTG TGA CGG GCG GTG TG

   

arcC-F

arcC

TTGATTCACCAGCGCGTATTGTC

450 bp

55°C

10

arcC-R

 

AGGTATCTGCTTCAATCAGCG

   

aroE-F

aroE

ATCGGAAATCCTATTTCACATTC

450 bp

55°C

10

aroE-R

 

GGTGTTGTATTAATAACGATATC

   

glpF-F

glpF

CTAGGAACTGCAATCTTAATCC

450 bp

55°C

10

glpF-R

 

TGGTAAAATCGCATGTCCAATTC

   

gmk-F

gmk

ATCGTTTTATCGGGACCATC

450 bp

55°C

10

gmk-R

 

TCATTAACTACAACGTAATCGTA

   

pta-F

pta

GTTAAAATCGTATTACCTGAAGG

450 bp

55°C

10

pta-R

 

GACCCTTTTGTTGAAAAGCTTAA

   

tpi-F

tpi

TCGTTCATTCTGAACGTCGTGAA

450 bp

55°C

10

tpi-R

 

TTTGCACCTTCTAACAATTGTAC

   

yqiL-F

yqiL

CAGCATACAGGACACCTATTGGC

450 bp

55°C

10

yqiL-R

 

CGTTGAGGAATCGATACTGGAAC