Relationship between tetracycline antibiotic susceptibility and genotype in oral cavity Lactobacilli clinical isolates

Table 3 SNPs and relative expression of mutant Tet-R genes in selected four clinical isolates and ATCC strains

Strains	Predicted Tet-R genes	Position in Reference	SNP	Relative expression of mutant Tet-R genes
B09/S23	tetPB	57,351	C to T	4.18 ± 1.58/3.87 ± 0.32
	tetPB	57,574	A to T	4.18 ± 1.58/3.87 ± 0.32
	tetT	19,518	G to A	0.32 ± 0.21/0.12 ± 0.04
		20,404	T to C
		20,503	A to G
		21,180	A to G
	otrA1	121,289	A to G	1.74 ± 0.23*/0.71 ± 0.17
		121,893	A to G
		122,217	A to G
		122,373	G to A
		122,475	A to G
	tetW1	201,420	A to C	7.9 ± 1.47*/1.54 ± 0.12
	tetW1	201,567	G to A	7.9 ± 1.47*/1.54 ± 0.12
	otrA2	217,467	C to T	0.90 ± 0.16/1.02 ± 0.21
	otrA2	217,786	G to A	0.90 ± 0.16/1.02 ± 0.21
	tetW2	86,983	A to G	0.53 ± 0.89/0.61 ± 0.12
	tcr3	116,527	G to A	0.49 ± 0.03/0.65 ± 0.15
	tcr3	117,173	A to G	0.49 ± 0.03/0.65 ± 0.15
B17	tetW	1,623,933	T to A	0.43 ± 0.12*
	tetO	1,636,215	G to A	0.21 ± 0.02*
		1,636,258	C to T
		1,636,464	C to G
		1,636,637	T to G
	tcr3	210,281	G to A	1.63 ± 3.10*
	tetT	1,850,771	T to C	0.13 ± 0.01*
	tetT	1,851,067	A to G	0.13 ± 0.01*
B43	tetT	89,875	G to A	1.21 ± 0.29
		90,659	C to A
		90,711	A to G
		90,814	G to A
		90,966	G to A
		91,116	T to C
	tetL	1,722,509	C to A	5.03 ± 0.95*
		1,722,558	A to C
		1,722,936	A to C
		1,722,984	G to A
	tetO	1,810,986	C to T	2.05 ± 0.49*
		1,811,028	C to G
		1,811,043	G to A
		1,811,052	G to A
	tcr3	1,178,583	G to A	4.99 ± 1.24*
		1,722,509	C to A
		1,722,558	A to C
		1,722,936	A to C
		1,722,984	G to A
	tetPB	2,686,361	T to A	2.84 ± 1.08*
		2,686,721	C to A
		2,686,722	G to A
		2,686,946	T to G
		2,686,952	C to T
		2,687,201	C to T
		2,687,423	G to A

* is represented the relative expression level of mutant Tet-R genes was significant difference between isolates and references strains.

ISSN: 2047-2994