Table 1 Primers for the detected Tet-R genes in selected clinical isolates and ATCC strains
Bacteria | Genes | Primers(5′-3′) | Amplified length (bp) |
|---|---|---|---|
L. fermentum | tetPB | F:AACTTACCCGATGGACTGGC | 146 |
R:CCCCAATCACTTCCCCGTTT | |||
tetT | F:CGACCCTATCCGAAGCCCTCT | 153 | |
R:GGGCGTATCTAGGAGGGTGAGTT | |||
otrA1 | F:GCCGTTACCGTTAGCATTAG | 195 | |
R:TAACTTAGCCAAAAAGGAGGGGATG | |||
otrA2 | F:ATAGCGGAGGCGTAAACTACTGGG | 166 | |
R:TAAAGAAGGCCCTGGAACAACAC | |||
tetW1 | F:ACGCTTTGGAGTTGGGATGT | 108 | |
R:GTTGTTCGTGGGTCCGCTCTT | |||
tetW2 | F:GAAGGTCAGGGCGGCGTCGTTT | 108 | |
R:ATGCAGCCAATGGTCTACGC | |||
tcr3 | F:GCGTTGGCTGAAGTAAAAGATGA | 177 | |
R:CCCCACAACGAAAACCCCCACTT | |||
L. rhamonsus | tetW | F:GCAAGACTGCGACTAACTTCATAAC | 161 |
R:GTTCTGGACGATATGGCACTTGA | |||
tetO | F:TGGCCTTCAATTCAAGCACATCT | 113 | |
R:AGACTGGGGTGGCGACACTATTT | |||
tcr3 | F: TAAGACGCCACTAAGCAGCAAAG | 131 | |
R:CCATTGTCGGCGGGTATCTGTTA | |||
tetT | F:ATCACATTCTTCGGGGTTACACG | 151 | |
R:TACAGCGGTAGCAGGGGACATTG | |||
L.plantarum | tetT | F:CATTTTGAACCGTTACGACACT | 260 |
R:GTCGCTTCACGAAAGTCACCACC | |||
tetO | F:CCTTTTCCACGGTCAAGACTAGC | 180 | |
R:TCTGATTCCTGAAGATTGGGGTG | |||
tetL | F:TAACAAGTAAGCCGTGGTCATCC | 123 | |
R:GGATTACTTTCATTTTGCGGGGT | |||
tcr3 | F:GTCTCATTTGTTGCCGACACTTC | 135 | |
R:TGTGCTGCCGTTTTTTGTGGTCC | |||
tetPB | F:ACTTGGCAAACAGCGGGGACT | 144 | |
R:CACTGACTTCATTAGCCATA | |||
|  | 16 s rRNA | F:CCTACGGGAGGCAGCAGTAG | 101 |
R:CAACAGAGCTTTACGATCCGAAA |