Fig. 3
Highlighter plot depicting nucleotide mismatches comparing the sequence of the strain obtained from patient C1 to all other RSV A strains, and the sequence of the strain obtained from patient C7 to all other RSV B strains. a RSV A and (b) RSV B glycoprotein sequences were aligned using MEGA and depicted as highlighter plot using the highlighter analysis tool [23]. Nucleotide exchanges compared to a reference sequence (C1_18_3 (1) for RSV A and C7_18_3 (1) for RSV B) are depicted in color. Absence of sequence information is depicted as grey bar. A schematic of the RSV G protein with the different domains is depicted on top [modified from [39]]. (1) indicates samples taken exclusively for strain typing at the 18th of March and (2) indicates samples collected for routine viral diagnostics. c RSV-G sequence alignment from other pediatric, non-outbreak patients compared to the references C1 and C7, respectively. d Sequencing chromatograms for RSV B cases. The depicted area is highlighted by * and ** in Figure 3B. Overlying sequence information from different quasispecies detected in the samples are highlighted in a box