Table 1 Primes used in this study
Gene | Sequence (5′–3′) | Product size (bp) | Annealing temperature (°C) |
|---|---|---|---|
gyrA | F: TACACCGGTCAACATTGAGG R: TTAATGATTGCCGCCGTCGG | 648 | 60 |
gyrB | F: CAAACTGGCGGACTGTCAGG R: TTCCGGCATCTGACGATAGA | 345 | 60 |
parC | F: GTCTGAACTGGGCCTGAATGC R: AGCAGCTCGGAATATTTCGACAA | 248 | 60 |
parE | F: ATGCGTGCGGCTAAAAAAGTG R: TCGTCGCTGTCAGGATCGATAC | 289 | 60 |
qnrA | F: ATTTCTCACGCCAGGATTTG R: GATCGGCAAAGGTTAGGTCA | 515 | 50 |
qnrB | F: GATCGTGAAAGCCAGAAAGG R: ACGATGCCTGGTAGTTGTCC | 468 | 54 |
qnrC | F: GGGTTGTACATTTATTGAATC R: TCCACTTTACGAGGTTCT | 446 | 50 |
qnrD | F: CGAGATCAATTTACGGGGAATA R: AACAAGCTGAAGCGCCTG | 581 | 50 |
qnrS | F: ACGACATTCGTCAACTGCAA R: TAAATTGGCACCCTGTAGGC | 416 | 55 |
aac(6′)-Ib-cr | F: GCAACGCAAAAACAAAGTTAGG R: GTGTTTGAACCATGTACA | 560 | 55 |
qepA | F: GCAGGTCCAGCAGCGGGTAG R: CTTCCTGCCCGAGTATCGTG | 217 | 60 |