Serotypes | Gene | Primer name | Primer Sequence (5'-3) | Size of product (bp) | PCR programs | M-PCR Volume (50 μL) |
---|---|---|---|---|---|---|
O1 | Wzx | wl-14632 | GTGAGCAAAAGTGAAATAAGGAACG | 1098 | 1 cycle: 95 °C ------------ 5 min. 30 cycle: 95 °C ------------ 30 s 62 °C ------------ 60 s 72 °C ------------ 60 s 1 cycle: 72 °C ------------ 5 min | 5 μL PCR buffer 10X 2.5 mM Mgcl2 300 μM dNTP (Fermentas) 0.4 μM of each primers F & R 2 U Taq DNA polymerase (Fermentas) 3 μL DNA template |
wl-14633 | CGCTGATACGAATACCATCCTAC | |||||
O6 | Wzy | wl-14646 | GGATGACGATGTGATTTTGGCTAAC | 783 | ||
wl-14647 | TCTGGGTTTGCTGTGTATGAGGC | |||||
O7 | Wzx | wl-14648 | CTATCAAAATACCTCTGCTGGAATC | 610 | ||
wl-14649 | TGGCTTCGAGATTAAACCTATTCCT | |||||
O8 | orf469 | wl-14652 | CCAGAGGCATAATCAGAAATAACAG | 448 | ||
wl-14653 | GCAGAGTTAGTCAACAAAAGGTCAG | |||||
O16 | Wzx | wl-14654 | GGTTTCAATCTCACAGCAACTCAG | 302 | ||
wl-14655 | GTTAGAGGGATAATAGCCAAGCGG | |||||
O21 | Wzx | wl-14676 | CTGCTGATGTCGCTATTATTGCTG | 209 | ||
wl-14677 | TGAAAAAAAGGGAAACAGAAGAGCC | |||||
O75 | Wzy | wl-17413 | GAGATATACATGGGGAGGTAGGCT | 511 | ||
wl-17414 | ACCCGATAATCATATTCTTCCCAAC | |||||
O2 | Wzy | wl-14636 | AGTGAGTTACTTTTTAGCGATGGAC | 770 | 1 cycle: 95 °C ------------ 5 min. 30 cycle: 94 °C ------------ 60 s 58 °C ------------ 60 s | 5 μL PCR buffer 10X 2.5 mM Mgcl2 300 μM dNTP (Fermentas) 0.4 μM of each primers F & R 2 U Taq DNA polymerase (Fermentas) |
wl-14637 | AGTTTAGTATGCCCCTGACTTTGAA | |||||
O4 | Wzx | wl-14642 | TTGTTGCGATAATGTGCATGTTCC | 664 | ||
wl-14643 | AATAATTTGCTATACCCACACCCTC | |||||
O15 | Wzy | wl-14672 | TCTTGTTAGAGTCATTGGTGTATCG | 183 | ||
wl-14673 | ATAAAACGAGCAAGCACCACACC | |||||
O18 | Wzx | wl-14656 | GTTCGGTGGTTGGATTACAGTTAG | 551 | ||
wl-14657 | CTACTATCATCCTCACTGACCACG | |||||
O22 | Wzx | wl-14660 | TTCATTGTCGCCACTACTTTCCG | 468 | ||
wl-14661 | GAAACAGCCCATGACATTACTACG | |||||
O25 | Wzy | wl-14666 | AGAGATCCGTCTTTTATTTGTTCGC | 230 | ||
wl-14667 | GTTCTGGATACCTAACGCAATACCC | |||||
O83 | Wzx | wl-14668 | GTACACCAGGCAAACCTCGAAAG | 362 | ||
wl-14669 | TTCTGTAAGCTAATGAATAGGCACC |