Intestinal carriage of methicillin-resistant Staphylococcus aureus in nasal MRSA carriers hospitalized in the neonatal intensive care unit

Nakao, Akihiro; Ito, Teruyo; Han, Xiao; Lu, Yu Jie; Hisata, Ken; Tsujiwaki, Atsushi; Matsunaga, Nobuaki; Komatsu, Mitsutaka; Hiramatsu, Keiichi; Shimizu, Toshiaki

doi:10.1186/2047-2994-3-14

Antimicrobial Resistance & Infection Control

Table 2 Characterization of the isolates using multiple PCR

From: Intestinal carriage of methicillin-resistant Staphylococcus aureus in nasal MRSA carriers hospitalized in the neonatal intensive care unit

Patients	Samples	Number of tested strains	Numbers of amplified DNA fragments^awith relate to
			ORFs^bon SCCmec elements		ORFs^con chromosome		ORFs^don mobile genetic elements
A	Nasal swab	4	IS1272 / type 2 ccr A	4 / 4	MW0919	4 / 4	SA1774 / SaGlm / SAV1998	4 / 4
	Feces	2	IS1272 / type 2 ccr A	2 / 2	MW0919	2 / 2	SA1774 / SaGlm / SAV1998	2 / 2
B	Nasal swab	4	IS1272 / type 2 ccr A	4 / 4	MW0919	4 / 4	SA1774 / SaGlm / SAV0881 / SA1801	4 / 4
	Feces	4	IS1272 / type 2 ccr A	4 / 4	MW0919	4 / 4	SA1774 / SaGlm / SAV0881 / SA1801	4 / 4
C	Nasal swab	4	mecI / type 2 ccrA /kdpC	4 / 4	SA2259	4 / 4	tnp B / SAV0898 / SAV0866 / SAV1974 / SAV0855 / SaGlm / SLTorf175 / SAV0913 / SLTorf182 / PV83orf2	4 / 4
	Feces	4	mecI / type 2 ccrA /kdpC	4 / 4	SA2259	4 / 4	tnp B / SAV0898 / SAV0866 / SAV1974 / SAV0855 / SaGlm / SLTorf175 / SAV0913 / SLTorf182 / PV83orf2	4 / 4
D	Nasal swab	4	mecI / type 2 ccrA /kdpC	4 / 4	SA2259	4 / 4	tnp B / SAV0898 / SAV0866 / SAV1974 / SAV0855 / SaGlm / SLTorf175 / SAV0913 / SLTorf182 / PV83orf2	4 / 4
	Feces	4	mecI / type 2 ccrA /kdpC	4 / 4	SA2259	4 / 4	tnp B / SAV0898 / SAV0866 / SAV1974 / SAV0855 / SaGlm / SLTorf175 / SAV0913 / SLTorf182 / PV83orf2	4 / 4
E	Nasal swab	2	IS1272 / type 2 ccr A	2 / 2	MW0919	2 / 2	SA1774 / SaGlm / SAV0881 / SA1801	2 / 2
	Feces	4	IS1272 / type 2 ccr A	4 / 4	MW0919	4 / 4	SA1774 / SaGlm / SAV0881 / SA1801	4 / 4
F	Nasal swab	4	IS1272 / type 2 ccr A	4 / 4	MW0919	4 / 4	tnp B / SAV0850 / SAV0898 / SAV0866 / SAV1974 / SAV0855 / SaGlm / SLTorf175 / PV83orf2	4 / 4
	Feces	4	IS1272 / type 2 ccr A	4 / 4	MW0919	4 / 4	tnp B / SAV0850 / SAV0898 / SAV0866 / SAV1974 / SAV0855 / SaGlm / SLTorf175 / PV83orf2	4 / 4
G	Nasal swab	3	IS1272 / type 2 ccr A	3 / 3	MW0919	3 / 3	tnp B / SaGlm / SLTorf182 / PV83orf2	2 / 3
G							tnp B / SAV0850 / SAV0898 / SAV0866 / SAV1974 / SAV0855 / SaGlm / SLTorf175 / PV83orf2	1 / 3
	Feces	4	IS1272 / type 2 ccr A	4 / 4	MW0919	4 / 4	tnp B / SaGlm / SLTorf182 / PV83orf2	4 / 4
H	Nasal swab	4	IS1272 / type 2 ccr A	4 / 4	MW0919	4 / 4	tnp B / SAV0898 / SAV0866 / SAV1974 / SAV0855 / SaGlm / SLTorf175 / SLTorf182 / PV83orf2	4 / 4
H	Feces	4	IS1272 / type 2 ccr A	4 / 4	MW0919	4 / 4	tnp B / SAV0898 / SAV0866 / SAV1974 / SAV0855 / SaGlm / SLTorf175 / SLTorf182 / PV83orf2	2 / 4
							tnp B / SAV0850 / SAV0898 / SAV0866 / SAV1974 / SAV0855 / SaGlm / SLTorf175 / SLTorf182 / PV83orf2	2 / 4
I	Nasal swab	4	IS1272 / type 2 ccr A	4 / 4	MW0919	4 / 4	tnp B / SaGlm / SLTorf182 / PV83orf2	4 / 4
	Feces	2	IS1272 / type 2 ccr A	2 / 2	MW0919	2 / 2	tnp B / SaGlm / SLTorf182 / PV83orf2	2 / 2
J	Nasal swab	4	IS1272 / type 2 ccr A	4 / 4	MW0919	4 / 4	SA1774 / SaGlm / SAV0881 / SA1801	4 / 4
	Feces	2	IS1272 / type 2 ccr A	2 / 2	MW0919	2 / 2	SA1774 / SaGlm / SAV0881 / SA1801	2 / 2
K	Nasal swab	4	IS1272 / type 2 ccr A	4 / 4	MW0919	4 / 4	tnp B / SAV0850 / SAV0898 / SAV0866 / SAV1974 / SAV0855 / SaGlm / SLTorf175 / SLTorf182 / PV83orf2	3 / 4
K							tnp B / SAV0898 / SAV0866 / SAV1974 / SAV0855 / SaGlm / SLTorf175 / SLTorf182 / PV83orf2	1 / 4
	Feces	2	IS1272 / type 2 ccr A	2 / 2	MW0919	2 / 2	tnp B / SAV0850 / SAV0898 / SAV0866 / SAV1974 / SAV0855 / SaGlm / SLTorf175 / SLTorf182 / PV83orf2	2 / 2
L	Nasal swab	1	IS1272 / type 2 ccr A	1 / 1	MW0919	1 / 1	tnp B/ SAV1774 / SaGlm / SAV0881 / SAV1801	1 / 1
	Feces	1	IS1272 / type 2 ccr A	1 / 1	MW0919	1 / 1	SAV0886 / SA1774 / SAV0885 / SaGlm / SAV0881 / SAV1801	1 / 1
M	Nasal swab	4	IS1272 / type 2 ccr A	4 / 4	MW0919	4 / 4	tnp B / SAV0850 / SAV0898 / SAV0866 / SAV1974 / SAV0855 / SaGlm / SLTorf175 / PV83orf2	4 / 4
	Feces	2	IS1272 / type 2 ccr A	2 / 2	MW0919	2 / 2	tnp B / SAV0850 / SAV0898 / SAV0866 / SAV1974 / SAV0855 / SaGlm / SLTorf175 / PV83orf2	2 / 2
N	Nasal swab	4	IS1272 / type 2 ccr A	4 / 4	MW0919	4 / 4	tnp B / SAV0850 / SAV0898 / SAV0866 / SAV1974 / SAV0855 / SaGlm / SLTorf175 / PV83orf2	4 / 4
	Feces	4	IS1272 / type 2 ccr A	4 / 4	MW0919	4 / 4	tnp B / SAV0850 / SAV0898 / SAV0866 / SAV1974 / SAV0855 / SaGlm / SLTorf175 / PV83orf2	4 / 4
O	Nasal swab	4	IS1272 / type 2 ccr A	4 / 4	MW0919	4 / 4	SA1774 / SaGlm / SAV0881 / SA1801	4 / 4
	Feces	4	IS1272 / type 2 ccr A	4 / 4	MW0919	4 / 4	SA1774 / SaGlm / SAV0881 / SA1801	4 / 4
P	Nasal swab	4	IS1272 / type 2 ccr A	4 / 4	MW0919	4 / 4	SA1774 / SaGlm / SAV0881 / SA1801	4 / 4
	Feces	4	IS1272 / type 2 ccr A	4 / 4	MW0919	4 / 4	SA1774 / SaGlm / SAV0881 / SA1801	4 / 4
Q	Nasal swab	4	IS1272 / type 2 ccr A	4 / 4	MW0919	4 / 4	SA1774 / SaGlm / SAV0881 / SA1801	4 / 4
	Feces	4	IS1272 / type 2 ccr A	4 / 4	MW0919	4 / 4	SA1774 / SaGlm / SAV0881 / SA1801	4 / 4
R	Nasal swab	4	IS1272 / type 2 ccr A	4 / 4	MW0919	4 / 4	SAV0898 / SAV1774 / SAV0855 / SaGlm / SLTorf175 / SAV0913 / SAV1998	4 / 4
	Feces	4	IS1272 / type 2 ccr A	4 / 4	MW0919	4 / 4	SAV0898 / SAV1774 / SAV0855 / SaGlm / SLTorf175 / SAV0913 / SAV1998	4 / 4
S	Nasal swab	4	mecI / type 2 ccrA /kdp C	4 / 4	SA2259	4 / 4	tnp B / SAV0898 / SAV0866 / SAV1774 / SAV0855 / SaGlm / SLTorf175 / SAV1801 / SAV0913 / SAV1998 / PV83orf2	4 / 4
	Feces	4	mecI / type 2 ccrA /kdp C	4 / 4	SA2259	4 / 4	tnp B / SAV0898 / SAV0866 / SAV1774 / SAV0855 / SaGlm / SLTorf175 / SAV1801 / SAV0913 / SAV1998 / PV83orf2	4 / 4
T	Nasal swab	4	IS1272 / type 2 ccr A	4 / 4	MW0919	4 / 4	SA1774 / SaGlm / SAV0881 / SA1801	4 / 4
	Feces	4	IS1272 / type 2 ccr A	4 / 4	MW0919	4 / 4	SA1774 / SaGlm / SAV0881 / SA1801	4 / 4
U	Nasal swab	4	IS1272 / type 2 ccr A	4 / 4	MW0919	4 / 4	SA1774 / SaGlm / SAV0881 / SA1801	4 / 4
	Feces	4	IS1272 / type 2 ccr A	4 / 4	MW0919	4 / 4	SA1774 / SaGlm / SAV0881 / SA1801	4 / 4

Abbreviations: ORFs open reading frames.
^aThe ORFs amplified by two multiplex PCR using the Cica Geneus Staph POT KIT are listed.
^bPCR-positive ORFs related to SCCmec elements. The results of PCR of five genes, mecA, mecI, IS1272, type 2 ccrA, and kdpC located in type IIa SCCmec, are listed. PCR-positive genes, except for mecA, are listed.
^cPCR-positive ORFs located on the chromosomes of N315(SA2259) and MW2(MW0919).
^dPCR-positive ORFs related to mobile genetic elements are listed. Fifteen ORFs were targeted for PCR, as follows: tnp B in the transposon Tn554, a ORF on the genome islands SaGIm, and 12 ORFs on the lysogenized bacteriophages (SA1774 and SA1801 for phi N315; SAV0881, SAV0850, SAV0855, SAV0866, SAV0881, SAV0898 and SAV0913 for phi Mu50A; SAV1974 and SAV1998 for phi Mu50B; orf 175 and orf 182 for phi SLT; and orf2 for phi PV83).

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ISSN: 2047-2994

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